Антитіла молока і сироватки крові людини: характеристика каталітичної активності та вплив на ріст і виживання клітин
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Namalwa, Jurkat, L1210 and L929 cell lines. We found anti-histone sIgA - and IgG-antibodies capable of catalyzing hydrolysis of linker histone H1 in the colostrums of healthy woman and blood serum of some patients with multiple sclerosis. Anti-histone IgG from blood serum of patients with multiple sclerosis were able to internalize into Jurkat and L929 cells and also to stimulate proliferation of Jurkat human T-cell line.
We also detected sIgA - and IgG-antibodies possessing lipid kinase and protein kinase activity in the collostrum of healthy woman and blood serum of some patients with system lupus erythrematosis. Lipids were co-purified with antibodies during their purification and were phosphorylated in those complexes in the presence of [-32P] ATP. Gel-filtration in dioxan containing buffer (5%) lead to dissociation of lipid-antibodies complexes and stimulation of protein kinase activity of immunoglobulins.
Keywords: antibodies, catalytic activity, cytotoxicity, proliferation, colostrum, blood serum, multiple sclerosis.Скачать